• Product NameEAAT3 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JU39-69
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein
• Alternative Names    EAA3_HUMAN antibody
  EAAC 1 antibody
  EAAC1 antibody
  EAAT 3 antibody
  Excitatory amino acid carrier 1 antibody
  Excitatory amino acid carrier1 antibody
  Excitatory amino acid transporter 3 antibody
  Excitatory amino acid transporter3 antibody
  Excitatory amino-acid carrier 1 antibody
  GLUTAMATE TRANSPORTER, HIGH-AFFINITY antibody
  MEAAC 1 antibody
  MEAAC1 antibody
  Neuronal and epithelial glutamate transporter antibody
  REAAC 1 antibody
  REAAC1 antibody
  Slc1 a1 antibody
  Slc1a 1 antibody
  SLC1A1 antibody
  Sodium dependent glutamate/aspartate transporter 3 antibody
  Sodium-dependent glutamate/aspartate transporter 3 antibody
  Solute carrier family 1 (neuronal / epithelial high affinity glutamate transporter, system Xag), member 1 antibody
  SOLUTE CARRIER FAMILY 1 (NEURONAL/EPITHELIAL HIGH AFFINITY GLUTAMATE TRANSPORTER), MEMBER 1 antibody
  Solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 antibody
  Solute carrier family 1 member 1 antibody
  Solute carrier family 1, member 1 antibody
  
• Accession No. Swiss-Prot#:P43005
• UniprotP43005
• Gene ID6505;
• Calculated MW 57 kDa (Predicted band size)
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500
  IHC: 1:50-1:200
  ICC/IF: 1:50-1:200
  
  

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Can also transport L-cysteine. Functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion. Mediates Cl- flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na+ symport. Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate. Negatively regulated by ARL6IP5.

• Western blot analysis of EAAT3 on mouse liver tissue lysate using anti-EAAT3 antibody at 1/500 dilution.
• Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.
• ICC staining EAAT3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining EAAT3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining EAAT3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting