• Product NameSEC23 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JU37-48
• PurificationProA affinity purified
• ApplicationsWB,ICC,IHC,FC
• Species ReactivityHu, Ms
• Immunogen DescriptionRecombinant protein
• Alternative Names    CLSD antibody
  Protein transport protein Sec23A antibody
  SC23A_HUMAN antibody
  Sec23 homolog A (S. cerevisiae) antibody
  SEC23-related protein A antibody
  sec23a antibody
  
• Accession No. Swiss-Prot#:Q15436
• UniprotQ15436
• Gene ID10484;
• Calculated MW 86/63 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex.

• Western blot analysis of SEC23 on NIH-3T3 cell using anti-SEC23 antibody at 1/500 dilution.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SEC23 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-SEC23 antibody. Counter stained with hematoxylin.
• ICC staining SEC23 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining SEC23 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of HepG2 cells with SEC23 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting