• Product NameNR0B1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JU33-05
• PurificationProA affinity purified
• ApplicationsWB,ICC,FC
• Species ReactivityHu
• Immunogen DescriptionRecombinant protein
• Alternative Names    AHC antibody
  AHCH antibody
  AHX antibody
  DAX 1 antibody
  DAX1 antibody
  Dosage sensitive sex reversal antibody
  DSS antibody
  DSS AHC critical region on the X chromosome protein 1 antibody
  DSS-AHC critical region on the X chromosome protein 1 antibody
  GTD antibody
  HHG antibody
  Nr0b1 antibody
  NR0B1_HUMAN antibody
  NROB1 antibody
  Nuclear hormone receptor antibody
  Nuclear receptor 0B1 antibody
  Nuclear receptor DAX 1 antibody
  Nuclear receptor DAX-1 antibody
  Nuclear receptor DAX1 antibody
  Nuclear receptor subfamily 0 group B member 1 antibody
  SRXY2 antibody
  
• Accession No. Swiss-Prot#:P51843
• UniprotP51843
• Gene ID190;
• Calculated MW 52 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500
  
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency.

• Western blot analysis of NR0B1 on A549 cell lysate using anti-NR0B1 antibody at 1/500 dilution.
• ICC staining NR0B1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining NR0B1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining NR0B1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of A549 cells with NR0B1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting