• Product NameDNA Ligase I Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JB43-39
• PurificationProA affinity purified
• ApplicationsWB,IHC
• Species ReactivityHu, Rt
• Alternative Names    DNA ligase 1 antibody
  DNA ligase I antibody
  DNLI1_HUMAN antibody
  LIG 1 antibody
  lig1 antibody
  Ligase I DNA ATP dependent antibody
  MGC117397 antibody
  MGC130025 antibody
  Polydeoxyribonucleotide synthase [ATP] 1 antibody
  
• Accession No. Swiss-Prot#:P18858
• UniprotP18858
• Gene ID3978;
• Calculated MW Predicted band size 102 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500
  IHC: 1:50-1:200
  
  
  

This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.

• Western blot analysis of DNA Ligase I on different cell lysates using anti-DNA Ligase I antibody at 1/500 dilution.
  Positive control:
  Lane 1: Daudi
  Lane 2: A431
  Lane 3: MCF-7
  
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-DNA Ligase I antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-DNA Ligase I antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-DNA Ligase I antibody. Counter stained with hematoxylin.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting