• Product NameWnt2b Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JB48-34
• PurificationProA affinity purified
• ApplicationsWB,IHC,ICC,IF,FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein
• Alternative Names    Protein Wnt-13 antibody
  Protein Wnt-2b antibody
  Wingless type MMTV integration site family, member 13 antibody
  Wingless type MMTV integration site family, member 2B antibody
  WNT13 antibody
  Wnt2b antibody
  WNT2B_HUMAN antibody
  XWNT2 antibody
  XWNT2, Xenopus, homolog of antibody
  
• Accession No. Swiss-Prot#:Q93097
• UniprotQ93097
• Gene ID7482;
• Calculated MW 44 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:50-1:200
  ICC: 1:100-1:500
  
  FC: 1:50-1:100

This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as carcinogenesis. Alternative splicing results in multiple transcript variants. Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a redundant role in embryonic lung development.

• Western blot analysis of Wnt2b on SiHa cell lysates using anti-Wnt2b at 1/500 dilution.
• Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.
• ICC staining Wnt2b in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Wnt2b in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Wnt2b in SHSY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of LOVO cells with Wnt2b antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting