• Product NameHistone H4 (acetyl K16) Rabbit mAb
• Clone No.JB21-44
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC,FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein
• Alternative Names    Histone gene cluster 1, H4A antibody
  Histone gene cluster 2, H4 antibody
  dJ160A22.1 antibody
  dJ160A22.2 antibody
  dJ221C16.1 antibody
  dJ221C16.9 antibody
  FO108 antibody
  H4 antibody
  H4 histone family, member A antibody
  H4 histone family, member B antibody
  H4 histone family, member C antibody
  H4 histone family, member D antibody
  H4 histone family, member E antibody
  H4 histone family, member G antibody
  H4 histone family, member H antibody
  H4 histone family, member I antibody
  H4 histone family, member J antibody
  H4 histone family, member K antibody
  H4 histone family, member M antibody
  H4 histone family, member N antibody
  H4 histone, family 2 antibody
  H4/A antibody
  H4/B antibody
  H4/C antibody
  H4/D antibody
  H4/E antibody
  H4/G antibody
  H4/H antibody
  H4/I antibody
  H4/J antibody
  H4/K antibody
  H4/M antibody
  H4/N antibody
  H4/O antibody
  H4/p antibody
  H4_HUMAN antibody
  H4F2 antibody
  H4F2iii antibody
  H4F2iv antibody
  H4FA antibody
  H4FB antibody
  H4FC antibody
  H4FD antibody
  H4FE antibody
  H4FG antibody
  H4FH antibody
  H4FI antibody
  H4FJ antibody
  H4FK antibody
  HIST1 cluster, H4A antibody
  HIST1 cluster, H4B antibody HIST1 cluster, H4D antibody HIST2H4 antibody Hist4 cluster, H4 antibody Hist4h4 antibody histone 1, H4a antibody histone 1, H4c antibody histone 1, H4d antibody histone 1, H4f antibody histone 1, H4h antibody histone 1, H4i antibody histone 1, H4j antibody histone 1, H4k antibody histone 1, H4l antibody histone 2, H4a antibody histone 2, H4b antibody Histone 4 family, member M antibody histone 4, H4 antibody histone cluster 1, H4 antibody histone cluster 1, H4a antibody histone cluster 1, H4b antibody histone cluster 1, H4c antibody histone cluster 1, H4d antibody histone cluster 1, H4e antibody histone cluster 1, H4f antibody histone cluster 1, H4h antibody histone cluster 1, H4i antibody histone cluster 1, H4j antibody histone cluster 1, H4k antibody histone cluster 1, H4l antibody histone cluster 2, H4a antibody histone cluster 2, H4b antibody histone cluster 4, H4 antibody Histone family, member A antibody Histone family, member B antibody Histone family, member D antibody Histone family, member H antibody Histone family, member I antibody Histone family, member L antibody Histone gene cluster 1, H4 antibody Histone gene cluster 1, H4D antibody Histone gene cluster 1, H4E antibody Histone gene cluster 1, H4K antibody Histone gene cluster 4, H4 antibody Histone gene cluster 4, H4 histone antibody Histone H4 antibody histone IV, family 2 antibody
• Accession No. Swiss-Prot#:P62805
• UniprotP62805
• Gene ID121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370;
• Calculated MW 11 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

• Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Histone H4 (acetyl K16) antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H4 (acetyl K16) antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Histone H4 (acetyl K16) antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H4 (acetyl K16) antibody. Counter stained with hematoxylin.
• ICC staining Histone H4 (acetyl K16) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Histone H4 (acetyl K16) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with Histone H4 (acetyl K16) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
• Western blot analysis of Histone H4 (acetyl K16) on SiHa cell using anti-Histone H4 (acetyl K16) antibody at 1/500 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting