• Product NameIRAK2 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JG36-23
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC,FC,IP
• Species ReactivityHu
• Immunogen DescriptionRecombinant protein within human IRAK2 aa 1-200.
• Alternative Names    il1rak2 antibody
  Interleukin 1 receptor associated kinase 2 antibody
  Interleukin 1 receptor associated kinase like 2 antibody
  Interleukin-1 receptor-associated kinase-like 2 antibody
  IRAK 2 antibody
  IRAK-2 antibody
  Irak2 antibody
  IRAK2_HUMAN antibody
  MGC150550 antibody
  
• Accession No. Swiss-Prot#:O43187
• UniprotO43187
• Gene ID3656;
• Calculated MW 69 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC/IF: 1:50-1:200
  IP: 1:10-1:50
  FC: 1:50-1:100

IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. IRAK2 has been shown to interact with TRAF6 and Myd88.

• Western blot analysis of IRAK2 on different cell lysates using anti-IRAK2 at 1/500 dilution.
  Positive control��
  Line 1: PC-3M
  Line 2: A549
  Line 3: HepG2
  
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.
• ICC staining IRAK2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Jurkat cells with IRAK2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting