• Product NameSF3B3 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JG63-53
• PurificationProA affinity purified
• ApplicationsWB,ICC,IHC,FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein within the C-terminus of human SF3B3.
• Alternative Names    KIAA0017 antibody
  Pre mRNA splicing factor SF3b 130 kDa subunit antibody
  Pre-mRNA-splicing factor SF3b 130 kDa subunit antibody
  RSE1 antibody
  SAP 130 antibody
  SAP130 antibody
  SF3b130 antibody
  SF3B3 antibody
  SF3B3_HUMAN antibody
  Spliceosome associated protein 130 antibody
  Spliceosome-associated protein 130 antibody
  Splicing factor 3b subunit 3 130kD antibody
  Splicing factor 3B subunit 3 antibody
  STAF130 antibody
  
• Accession No. Swiss-Prot#:Q15393
• UniprotQ15393
• Gene ID23450;
• Calculated MW 136 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:50-1:200
  ICC: 1:50-1:100
  
  FC: 1:50-1:100

SF3B is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3B contains the spliceosomal proteins SAP 49, SAP 130 (also known as SF3B3), SAP 145 and SAP 155. SF3B3, SAP 145 and SAP 155 are present in a protein complex in HeLa nuclear extracts and associate with one another. While SF3B3 and SAP 155 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.

• Western blot analysis of SF3B3 on Jurkat and Hela cells lysates using anti-SF3B3 antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.
• ICC staining SF3B3 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of LOVO cells with SF3B3 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting