• Product NameSOCS2 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JG36-86
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IP
• Species ReactivityHu
• Immunogen DescriptionRecombinant protein within human SOCS2 1-150aa.
• Alternative Names    CIS 2 antibody
  CIS-2 antibody
  CIS2 antibody
  Cish 2 antibody
  Cish2 antibody
  Cytokine inducible SH2 protein 2 antibody
  Cytokine-inducible SH2 protein 2 antibody
  SOCS 2 antibody
  SOCS-2 antibody
  Socs2 antibody
  SOCS2_HUMAN antibody
  SSI 2 antibody
  SSI-2 antibody
  SSI2 antibody
  STAT induced STAT inhibitor 2 antibody
  STAT-induced STAT inhibitor 2 antibody
  STATI 2 antibody
  STATI2 antibody
  Suppressor of cytokine signaling 2 antibody
  
• Accession No. Swiss-Prot#:O14508
• UniprotO14508
• Gene ID8835;
• Calculated MW 22 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  
  ICC: 1:50-1:200
  IP: 1:10-1:50
  

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

• Western blot analysis of SOCS2 on different lysates using anti-SOCS2 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Human placenta
  Lane 2: SH-SY-5Y
  
• ICC staining SOCS2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining SOCS2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining SOCS2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting