• Product NameNucleostemin Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JG37-82
• PurificationProA affinity purified
• ApplicationsWB,ICC,IHC,FC
• Species ReactivityHu
• Immunogen DescriptionRecombinant protein corresponding to the C-terminus of human Nucleostemin.
• Alternative Names    C77032 antibody
  E2 induced gene 3 protein antibody
  E2-induced gene 3 protein antibody
  E2IG3 antibody
  Estradiol induced nucleotide binding protein antibody
  GNL3 antibody
  GNL3_HUMAN antibody
  Guanine nucleotide binding protein like 3 (nucleolar) antibody
  Guanine nucleotide binding protein like 3 antibody
  Guanine nucleotide-binding protein-like 3 antibody
  MGC800 antibody
  NNP47 antibody
  Novel nucleolar protein 47 antibody
  NS antibody
  Nucleolar GTP binding protein 3 antibody
  Nucleolar GTP-binding protein 3 antibody
  Nucleostemin antibody
  Putative nucleotide binding protein estradiol induced antibody
  
• Accession No. Swiss-Prot#:Q9BVP2
• UniprotQ9BVP2
• Gene ID26354;
• Calculated MW 62 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• 
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene.

• Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Nucleostemin antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Nucleostemin antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Nucleostemin antibody. Counter stained with hematoxylin.
• ICC staining Nucleostemin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Nucleostemin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of A549 cells with Nucleostemin antibody at 1/50 dilution (Fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting