• Product NameAnnexin IV Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JG37-83
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC,FC
• Species ReactivityHu
• Immunogen DescriptionRecombinant protein within human Annexin IV aa 1-200.
• Alternative Names    35 beta calcimedin antibody
  35-beta calcimedin antibody
  AIV antibody
  Annexin 4 antibody
  Annexin A4 antibody
  Annexin IV (placental anticoagulant protein II) antibody
  Annexin IV antibody
  Annexin IV placental anticoagulant protein II antibody
  Annexin-4 antibody
  AnnexinA4 antibody
  AnnexinIV antibody
  ANX 4 antibody
  ANX A4 antibody
  ANX4 antibody
  ANXA 4 antibody
  ANXA4 antibody
  ANXA4 protein antibody
  ANXA4_HUMAN antibody
  Carbohydrate binding protein P33/P41 antibody
  Carbohydrate-binding protein p33/p41 antibody
  Chromobindin 4 antibody
  Chromobindin-4 antibody
  Chromobindin4 antibody
  DKFZp686H02120 antibody
  Endonexin I antibody
  EndonexinI antibody
  Lipocortin IV antibody
  LipocortinIV antibody
  MGC75105 antibody
  OTTHUMP00000160052 antibody
  OTTHUMP00000202207 antibody
  P32.5 antibody
  P33/41 antibody
  PAP II antibody
  PAP-II antibody
  PAPII antibody
  PIG 28 antibody
  PIG28 antibody
  Placental anticoagulant protein II antibody
  PP4 X antibody
  PP4-X antibody
  PP4X antibody
  Proliferation inducing gene 28 antibody
  Proliferation inducing protein 28 antibody
  Protein II antibody
  Xanx-4 antibody
  ZAP 36 antibody
  ZAP36 antibody
  
• Accession No. Swiss-Prot#:P09525
• UniprotP09525
• Gene ID307;
• Calculated MW 36 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC/IF: 1:50-1:200
  
  FC: 1:50-1:100

The annexin family of calcium-binding proteins is composed of at least ten mammalian genes. It is characterized by a conserved core domain which binds to phospholipids in a Ca++-dependent manner and a unique amino terminal region which may confer binding specificity. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. For example, the crystal structure of Annexin III has suggested a hydrophilic amino terminus with possible Ca++ channel activity. Similarly, Annexin V has ion channel properties. Annexin IV, also referred to as endonexin, functions to regulate Cl- flux by mediating calmodulin kinase II (CaMKII) activity and Annexin V has been shown to regulate PKC activity.

• Western blot analysis of Annexin IV on human liver tissue lysate using anti-Annexin IV antibody at 1/2,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Annexin IV antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Annexin IV antibody. Counter stained with hematoxylin.
• ICC staining Annexin IV in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Annexin IV in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Annexin IV in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of HepG2 cells with Annexin IV antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting