• Product NameVAMP1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JG40-19
• PurificationProA affinity purified
• ApplicationsWB,IHC,ICC,IF
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionFull length recombinant protein of human VAMP1.
• Alternative Names    DKFZp686H12131 antibody
  SYB 1 antibody
  SYB1 antibody
  Synaptobrevin 1 antibody
  Synaptobrevin-1 antibody
  Synaptobrevin1 antibody
  VAMP 1 antibody
  VAMP-1 antibody
  Vamp1 antibody
  VAMP1_HUMAN antibody
  Vesicle associated membrane protein 1 antibody
  Vesicle associated membrane protein 1 synaptobrevin 1 antibody
  Vesicle-associated membrane protein 1 antibody
  
• Accession No. Swiss-Prot#:P23763
• UniprotP23763
• Gene ID6843;
• Calculated MW 13 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC/IF: 1:50-1:200
  
  

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined.

• Western blot analysis of VAMP1 on different tissue lysates using anti-VAMP1 antibody at 1/2,000 dilution.
  Positive control:
  Lane 1: Mouse cerebellum
  Lane 2: Rat brain
  
• Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.
• ICC staining VAMP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining VAMP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining VAMP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting