• Product NameAconitase 2 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JE40-83
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC,IP
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein within human Aconitase 2 aa 1-150.
• Alternative Names    ACO 2 antibody
  ACO2 antibody
  ACON_HUMAN antibody
  aconitase 2 antibody
  Aconitase 2 mitochondrial antibody
  Aconitase antibody
  Aconitase2 antibody
  Aconitate hydratase antibody
  Aconitate hydratase mitochondrial antibody
  ACONM antibody
  Citrate hydro lyase antibody
  Citrate hydro-lyase antibody
  ICRD antibody
  mitochondrial antibody
  
• Accession No. Swiss-Prot#:Q99798
• UniprotQ99798
• Gene ID50;
• Calculated MW 85 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  IP: 1:10-1:50
  

Aconitase 2, mitochondrial is a protein that in humans is encoded by the ACO2 gene. The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15 (PRSS15), also known as Lon protease, after oxidative modification.

• Western blot analysis of Aconitase 2 on SiHa (1) and HL-60 (2) cell lysate using anti-Aconitase 2 antibody at 1/2,000 dilution.
• Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Aconitase 2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Aconitase 2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Aconitase 2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Aconitase 2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Aconitase 2 antibody. Counter stained with hematoxylin.
• ICC staining Aconitase 2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting