• Product NameBAF57 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JE41-11
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC,IP
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein within human BAF57 aa 1-200.
• Alternative Names    BAF57 antibody
  BRG1 associated factor 57 antibody
  BRG1-associated factor 57 antibody
  Chromatin remodeling complex BRG1 associated factor 57 antibody
  FLJ35648 antibody
  SMARCE 1 antibody
  SMARCE1 antibody
  SMCE1_HUMAN antibody
  SWI/SNF related matrix associated actin dependent regulator of chromatin e1 antibody
  SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1 antibody
  SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 antibody
  
• Accession No. Swiss-Prot#:Q969G3
• UniprotQ969G3
• Gene ID6605;
• Calculated MW 47 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC/IF: 1:50-1:200
  IP: 1:50-1:200
  

The SWI/SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF1 or SNF2α) and Brg-1 (also designated SNF2 or SNF2β) are the ATPase subunits of the mammalian SWI/SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI/SNF complex. In higher eukaryotes, BAF57 is also a critical component of the SWI/ SNF complex. BAF57 contains a high-mobility-group (HMG) domain adjacent to a kinesin-like region and is a DNA-binding subunit of the SWI/SNF complex. The human BAF57 gene maps within the q12-25 region of chromosome 17, a gene-rich area implicated in breast and ovarian cancers.

• Western blot analysis of BAF57 on SH-SY-5Y cell lysate using anti-BAF57 antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-BAF57 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using anti-BAF57 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-BAF57/SMARCE1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BAF57 antibody. Counter stained with hematoxylin.
• ICC staining BAF57 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining BAF57 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining BAF57 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting