Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate YBX1 in samples. An antibody specific for YBX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYBX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YBX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YBX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Y box binding protein 1is a human protein. Current research is examining its involvement in cancer, and particularly in the metastasis of cancerous cells or prevention thereof.
The deduced 322-amino acid protein contains 4 putative DNA-binding domains, 3 of which are rich in basic amino acids. Two of these basic regions, basic-1 and basic-2, form the double-strand DNA-binding domain of the protein. NSEP1 also has a pro/ser/thr-rich domain and an asp/glu/gln-rich domain, both of which are reminiscent of activation domains. It also contains an octapeptide single-strand DNA-binding motif that shares weak homology with the ribonucleoprotein consensus sequence of RNA-binding proteins.
