Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate WNT1 in samples. An antibody specific for WNT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWNT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The WNT gene family consists of structurally related genes which encode secreted signaling proteins. WNT1 is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level.
The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region.
