Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate WDR52 in samples. An antibody specific for WDR52 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWDR52 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WDR52 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WDR52 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:WDR53 Contains5 WD repeats. WD-repeat proteins belong to a large and fast-expanding conservative protein family. As demonstrated by the crystal structure of the G protein beta subunit, all WD-repeat proteins are speculated to form a circularized beta propeller structure. The importance of these proteins is not only demonstrated by their critical roles in many essential biological functions ranging from signal transduction, transcription regulation, to apoptosis, but is also recognized by their association with several human diseases. Defining the function of a WD-repeat protein is the current challenge. It is, however, paramount to uncover the function of individual WD-repeat proteins, explore the protein interaction mechanism through WD-repeat domains and, ultimately, understand the complex biological processes and organisms themselves.
