Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate VEGFC in samples. An antibody specific for VEGFC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVEGFC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGFC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGFC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Vascular endothelial growth factor receptor-1 was originally discovered through the screening of a human placental cDNA library . It is a receptor tyrosine kinase (RTK) specific for the angiogenic factors VEGF (VEGF-A), PlGF, and VEGF-B. VEGF R1 is expressed in two forms via alternate splicing at the pre-mRNA level: a full-length, membrane bound receptor capable of transducing signal, and a truncated, soluble receptor (sVEGF R1) capable of sequestering ligand or dimerizing with full-length receptor and preventing signal transduction.
Although VEGF R1 null mutations are lethal, deletions of the kinase domain are not, suggesting that the soluble form, or at least the extracellular domain, is all that is necessary for normal vascular development.
