Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate VD2 in samples. An antibody specific for VD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Ergocalciferol is a provitamin form of vitamin D, also called vitamin D2. Ergocalciferol is created when ultraviolet light activates ergosterol. Ergocalciferol is produced in fungi synthetically through irradiation of ergosterol. Human bioavailability of vitamin D2 from vitamin D2-enhanced button mushrooms via UV-B irradiation is effective in improving vitamin D status and not different from a vitamin D2 supplement. Vitamin D2 from UV-irradiated yeast baked into bread is bioavailable. By visual assessment or using a chromometer, no significant discoloration of irradiated mushrooms, as measured by the degree of "whiteness", was observed. One study found low vitamin D2 levels in patients with Alzheimer's disease, but this observational study did not prove cause or effect related to ergocalciferol and vitamin D2 deficiency in the diet.
