Detection Method:competitive
Test principle:This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VB12. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated VB12 and incubated. The competitive inhibition reaction is launched between with HRP labeled VB12 and unlabeled VB12 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of VB12 in the sample. The color development is stopped and the intensity of the color is measured. Product Overview:Vitamin B12, vitamin B12 or vitamin B-12, also called cobalamin, is a water- soluble vitamin with a key role in the normal functioning of the brain and nervous system, and for the formation of blood. It is one of the eight B vitamins. It is normally involved in the metabolism of every cell of the human body, especially affecting DNA synthesis and regulation, but also fatty acid metabolism and amino acid metabolism. Neither fungi, plants, nor animals (including humans) are capable of producing vitamin B12. Only bacteria and archaea have the enzymes required for its synthesis, although many foods are a natural source of B12 because of bacterial symbiosis. The vitamin is the largest and most structurally complicated vitamin and can be produced industrially only through bacterial fermentation-synthesis.
