Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate VASH1 in samples. An antibody specific for VASH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVASH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VASH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VASH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:VASH2 deduced 355-amino acid protein shares 52.5% amino acid identity with VASH1 and 97.5% amino acid identity with mouse Vash2. VASH2 expression at embryonic weeks 6 to 12 in human heart, brain, kidney, lung, muscle, and whole embryo. RT-PCR analysis detected low levels of VASH2 expression in HUVECs and aortic endothelial cells. VASH2 expression in cell lines was not inducible by VEGF, FGF2, or PMA, all of which can induce VASH1 expression.
However, in an in vivo mouse model, Vash2 was induced in embryonic brain transfected with VEGF. Immunohistochemistry showed that VASH2 and VASH1 were present in vessels from 20-week human embryonic lung.VASH2 has antiangiogenic activity. In addition, VASH2 inhibited network formation by HUVECs.
