Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate VAMP2 in samples. An antibody specific for VAMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVAMP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VAMP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VAMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:VAMP2 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP2 is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG.
VAMP2 is thought to participate in neurotransmitter release at a step between docking and fusion. Mice lacking functional synaptobrevin2/VAMP2 gene cannot survive after birth, and have a dramatically reduced synaptic transmission, around 10% of control. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and complexin. It also forms a distinct complex with synaptophysin.
