Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate VA in samples. An antibody specific for VA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The content of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) in endosomal membranes changes dynamically with fission and fusion events that generate or absorb intracellular transport vesicles. VAC14 is a component of a trimolecular complex that tightly regulates the level of PtdIns(3,5)P2. Other components of this complex are the PtdIns(3,5)P2-synthesizing enzyme PIKFYVE and the PtdIns(3,5)P2 phosphatase FIG4 . VAC14 functions as an activator of PIKFYVE. The deduced 221-amino acid TRX protein is predominantly hydrophilic in its C-terminal region. Northern blot analysis of human tissues detected a major 3.5-kb TRX transcript in all tissues examined, namely brain, heart, lung, liver, pancreas, kidney, skeletal muscle, and placenta. However, Western blot analysis detected TRX expression only in lymphocytes and lymphocytic-derived cell lines.
