Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate UTS2 in samples. An antibody specific for UTS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUTS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UTS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UTS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Urotensin-II (U-II) is a peptide ligand, initially isolated from the neurosecretory system of the Goby fish (Gillichthys mirabilis) Bern et al. 1969. For many years it was thought that U-II does not exhibit significant effects in mammalian systems; a view quickly overturned when it was demonstrated that Goby U-II produces slow relaxation of mouse annococygeus muscle, in addition to contraction of rat artery segments. In 1998, the cDNA encoding a U-II precursor was cloned in humans, unequivocally demonstrating its existence in mammalian species.As with other peptide ligands, U-II is synthesised from a larger precursor molecule, known as Prepro-urotensin-II, two isoforms have been identified in man of lengths 124 and 139 residues. Cleavage of either of these precursors produces identical, eleven residue, mature U-II peptides.
