Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate USP8 in samples. An antibody specific for USP8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUSP8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for USP8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of USP8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Ubiquitin, a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains.
During analysis of randomly sampled human coding sequences from the myeloid cell line KG-1, Nomura et al. (1994) identified a novel cDNA, which they referred to as KIAA0055, that was related to the tre2 oncogene. Janssen et al. (1998) found that HUMORF8 encodes a putative deubiquitinating enzyme. Janssen et al. (1998) analyzed DNA from a patient with chronic myeloproliferative disorder. They identified an oncogenic fusion of the 5-prime end of p85-beta with the 3-prime end of HUMORF8.
