Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate UQCRC2 in samples. An antibody specific for UQCRC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUQCRC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UQCRC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UQCRC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The amount of PPARG, PPARG coactivator-1-alpha (PPARGC1A), ubiquinol-cytochrome c oxidoreductase core 2 subunit (UQCRC2), cytochrome c oxidase subunit I (MTCO1), uncoupling protein-2 (UCP2), and ATP synthase H(+)-transporting mitochondrial F1 complex (F1-ATP synthase) were markedly reduced in the low capacity runner rats in comparison with the high capacity runners.
The uniform decline in these proteins was consistent with the hypothesis that reduced aerobic metabolism plays a causal role in the development of the differences between the low capacity runner and high capacity runner rats. impairment of mitochondrial function may link reduced fitness to cardiovascular and metabolic disease.
Organism species: Homo sapiens (Human)
