Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate UQCR in samples. An antibody specific for UQCR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUQCR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UQCR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UQCR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:UQCRB is a subunit of the respiratory chain protein Ubiquinol Cytochrome c Reductase (UQCR,Complex III or Cytochrome bc1 complex), Subunit 7 was identified as a Q-binding protein by photo-labeling with a ubiquinone analog (subsequent structures show it to be exposed to the lipid phase but not involved in either Q-binding site). Subunits 6 and 7 reverse position on going from Laemli gels to Weber&Osborne gels, and one might suspect the name "Q-binding protein" arose from confusion with subunit 7. I have been told however that this is not the case, and both proteins were separately identified as Q-binding proteins. Genome anotators improved the situation by naming its gene "UQCR binding", UQCRB.
