Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate UPRT in samples. An antibody specific for UPRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUPRT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UPRT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UPRT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Uqcc, called Bfzb, and identified its human homolog by database analysis. The deduced human and mouse proteins have a single transmembrane domain and share 94% amino acid identity. In situ hybridization of mouse embryos detected Bfzb predominantly in the developing nervous system, with highest expression in proliferating neuroepithelia of brain and neural tube, ganglia of cranial nerves V, VII, VIII, IX, and X, and dorsal root ganglia.
Transcripts were also detected in developing eye and brown fat. In adult mouse brain, strong expression was restricted to olfactory bulb, hippocampus, and piriform cortex and Purkinje cells of cerebellum. The Bfzb protein localized to cytoplasmic vesicular structures.
