Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate UGT1A1 in samples. An antibody specific for UGT1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUGT1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UGT1A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UGT1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:UGT1A10 encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The enzyme encoded by this gene has glucuronidase activity on mycophenolic acid, coumarins, and quinolines.
