Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TYMS in samples. An antibody specific for TYMS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTYMS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TYMS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TYMS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Tyrosinase (EC 1.14.18.1) catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. Kwon et al. screened a lambda-gt11 human melanocyte cDNA library with antibodies against hamster tyrosinase to isolate a clone for human tyrosinase. The deduced 548-amino acid protein has a molecular mass of 62,610 Da and contains glycosylation sites and histidine-rich sites that could be used for copper binding.
OCA1 is the gene associated with albinism, an autosomal recessive disorder. Albinism occurs when something causes the OCA1 gene to function improperly. The locus of gene OCA1 may be written "11q1.4-q2.1", meaning it is on the long arm of chromosome 11, somewhere in the range of sub-band 4 of band 1, and sub-band 1 of band 2.
