Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TYMS in samples. An antibody specific for TYMS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTYMS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TYMS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TYMS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Thymidylate synthetase (EC 2.1.1.45) is the enzyme used to generate thymidine monophosphate (dTMP), which is subsequently phosphorylated to thymidine triphosphate for use in DNA synthesis and repair. By means of reductive methylation, deoxyuridine monophosphate (dUMP) and N5,N10-methylene tetrahydrofolate are together used to form dTMP, yielding dihydrofolate as a secondary product.
As an anti-cancer chemotherapy target, thymidylate synthetase can be inhibited by the thymidylate synthase inhibitors such as fluorinated pyrimidine fluorouracil, or certain folate analogues, including most notably raltitrexed (trade name Tomudex).
