Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TXNRD2 in samples. An antibody specific for TXNRD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTXNRD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TXNRD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TXNRD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Thioredoxin reductase (TR) is a dimeric NADPH-dependent FAD containing enzyme that catalyzes the reduction of the active site disulfide of thioredoxin and other substrates. TR is a member of a family of pyridine nucleotide-disulfide oxidoreductases and is a key enzyme in the regulation of the intracellular redox environment. Three thioredoxin reductase genes have been found that encode selenocysteine containing proteins.
TrxR2 partially overlaps the COMT gene on chromosome 22.Highly expressed in the prostate, ovary, liver, testis, uterus, colon and small intestine. Intermediate levels in brain, skeletal muscle, heart and spleen. Low levels in placenta, pancreas, thymus and peripheral blood leukocytes.
