• Product NameVCP(p97) antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 176 of VCP(p97)
• Target NameVCP(p97)
• Accession No. Swiss-Prot:P55072
  Gene ID:7415
• UniprotP55072
• Gene ID7415;
• Concentration 0.3 mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 89kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:500
  Immunofluorescence: 1:100-1:200

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: Hela
  B: Hep G2
  C: Molt-4
  D: Raji
  7.5% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97) antibody at 1: 500 dilution.
• Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using VCP(p97) antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting