Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TRPM2 in samples. An antibody specific for TRPM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI).This protein was first identified in 1988 in seminal fluid.In humans the gene is encoded on chromosome 8 (8p21) and is highly conserved between species (70-80% homology).It is expressed in most mammalian tissues and can be found in blood plasma, milk, urine, cerebrospinal fluid and semen. A number of proteins have been found to affect its expression including Egr-1, members from the AP-1 complex, HSF1/2, Cdx1/2 and B-Myb.
