Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TRAK2 in samples. An antibody specific for TRAK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRAK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRAK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRAK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:ALS2CR3 contains a coiled-coil domain that shares significant homology with the coiled-coil region in huntingtin-associated protein-1 (HAP1). Northern blot analysis revealed ubiquitous expression of 7.0- and 4.5-kb transcripts, reflecting the use of 2 alternate polyadenylation signals; expression was highest in heart.
Northern blot analysis detected a major 6.2-kb transcript in all tissues tested except testis, which expressed a truncated transcript of 2.9 kb; a minor 4.2-kb transcript was also detected. Western blot analysis revealed tissue-specific expression of 3 Grif1 species with different molecular masses in brain, heart, and skeletal muscle. Grif1 was expressed in HEK293 cells as 3 proteins with apparent molecular masses of 98, 106, and 115 kD.
