Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TPT1 in samples. An antibody specific for TPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:TCTP, is an essential component of the tuberous sclerosis-RHEB pathway. Reducing Drosophila Tctp levels reduced cell size, cell number, and organ size, which mimics Drosophila Rheb mutant phenotypes. Drosophila Tctp is genetically epistatic to Tsc1 and Rheb, but acts upstream of S6K, a downstream target of Rheb. Drosophila Tctp directly associated with Rheb and displayed guanine nucleotide exchange activity with it in vivo and in vitro. Human TCTP showed similar biochemical properties compared to Drosophila Tctp and could rescue Drosophila Tctp mutant phenotypes, suggesting that the function of TCTP in the TSC pathway is evolutionarily conserved. Overexpression of TSAP6 increased the level of TPT1 in exosome preparations and consistently enhanced TPT1 secretion.
