Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TPSAB1 in samples. An antibody specific for TPSAB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPSAB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPSAB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPSAB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors.
TPSb2 is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, beta II and beta III. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
