Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TOM1L1 in samples. An antibody specific for TOM1L1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOM1L1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOM1L1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOM1L1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Mice hypomorphic for Tom1l2 exhibited numerous infections and tumors compared to wild-type littermates. Associated with this increased risk for infection and tumor formation, apparently healthy Tom1l2 hypomorphs also had splenomegaly, elevated B- and T-cell counts, and an impaired humoral response, although at a reduced penetrance.
Furthermore, cellular localization studies showed that a Tom1l2-GFP fusion protein colocalizes with Golgi compartments, supporting the role of Tom1l2 in cellular trafficking, while molecular modeling and bioinformatic analysis of Tom1l2 illustrated a structural basis for a functional role in trafficking. These results indicate a role for Tom1l2 in the immune response and possibly in tumor suppression.
