Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TNPO3 in samples. An antibody specific for TNPO3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNPO3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNPO3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNPO3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:TNPO3 is a nuclear import receptor for serine/arginine-rich (SR) proteins, which are essential precursor-mRNA splicing factors.By yeast 2-hybrid analysis using the RS domain of the E2 protein of human papillomavirus (HPV)-5 as bait, Lai et al. (2000) cloned a TNPO3 splice variant, TRNSR2, from a HeLa cell cDNA library. The deduced 923-amino acid TRNSR2 protein lacks 2 regions of about 30 amino acids each found in the TRNSR protein identified by Kataoka et al. (1999). Northern blot analysis detected ubiquitous expression of a 4.5-kb transcript, with highest expression in testis. Epitope-tagged TRNSR2 localized throughout transfected HeLa cells, but a mutant lacking the N-terminal region colocalized with splicing factor SC35 (SFRS2) in nuclear speckles.
