Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TNKS1BP1 in samples. An antibody specific for TNKS1BP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNKS1BP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNKS1BP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNKS1BP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:TNKS1BP1, has a calculated molecular mass of 182 kD. It is predicted to contain 2 basic regions at its N and C termini and a large internal acidic region. The C terminus also contains 2 potential nuclear localization signals. Northern blot analysis detected transcripts of 7, 4.4, and 2.3 kb in multiple tissues, with highest expression in testis, ovary, and lung, and minimal expression in brain and peripheral blood leukocytes. By immunolocalization of endogenous TAB182 in 2 human cell lines, Seimiya and Smith (2002) found TAB182 in the cytoplasm, where it colocalized with cortical actin, and in the nucleus. The nuclear staining was similar to that found for heterochromatin proteins. In a synchronized population of HeLa cells, TAB182 associated with chromosomes throughout mitosis.
