Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TNFSF13B in samples. An antibody specific for TNFSF13B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF13B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF13B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF13B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:B-cell activating factor (BAFF) is the tumor necrosis factor ligand superfamily member 13B (TNFLSF13B), also known as B Lymphocyte Stimulator (BLyS) and TNF- and APOL-related leukocyte expressed ligand (TALL-1) and the Dendritic cell-derived TNF-like molecule (CD257 antigen; cluster of differentiation 257). BAFF is a 285-amino acid long peptide glycoprotein which undergoes glycosylation at residue 124. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. The transmembrane form can be cleaved from the membrane, generating a soluble protein fragment. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it.
