Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TMTC3 in samples. An antibody specific for TMTC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMTC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMTC3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMTC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:TMTC3 encodes a protein that belongs to the transmembrane and tetratricopeptide repeat-containing protein family. The tetratrico peptide repeat (TPR) is a structural motif present in a wide range of proteins. It mediates protein-protein interactions and the assembly of multiprotein complexes. The TPR motif consists of 3-16 tandem-repeats of 34 amino acids residues, although individual TPR motifs can be dispersed in the protein sequence. Sequence alignment of the TPR domains reveals a consensus sequence defined by a pattern of small and large amino acids. TPR motifs have been identified in various different organisms, ranging from bacteria to humans. Proteins containing TPRs are involved in a variety of biological processes, such as cell cycle regulation, transcriptional control, mitochondrial and peroxisomal protein transport, neurogenesis and protein folding.
