Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TMOD1 in samples. An antibody specific for TMOD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMOD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMOD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMOD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:TMOD1 encodes a member of the tropomodulin family. The encoded protein is an actin-capping protein that regulates tropomyosin by binding to its N-terminus, inhibiting depolymerization and elongation of the pointed end of actin filaments and thereby influencing the structure of the erythrocyte membrane skeleton.
TMOD gene is expressed in major human tissues at different levels in the following order: heart and skeletal muscle much greater than in brain, lung, and pancreas, which is greater than in placenta, liver, and kidney. They pointed to structural similarities between tropomodulin and the 64-kD autoantigen in Graves disease and suggested that the 2 genes evolved from a common ancestral gene.
