Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TMEM176A in samples. An antibody specific for TMEM176A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM176A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM176A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM176A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:By serologic analysis of recombinant cDNA expression libraries (SEREX) from 4 hepatocellular carcinoma patients, Wang et al. (2002) cloned HCA112. The deduced protein contains 235 amino acids. RT-PCR detected HCA112 expression in human kidney, lung, and pancreas, but not in any other tissue examined.
Nakajima et al. (2002) cloned the mouse homolog of HCA112, Gs188. The deduced 244-amino acid protein has a calculated molecular mass of 26.6 kD and contains 4 putative hydrophobic domains. Northern blot analysis detected Gs188 expression in mouse kidney, lung, and spleen; little to no expression was detected in other tissues examined.
