Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TMEFF1 in samples. An antibody specific for TMEFF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEFF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEFF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEFF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:EGF-like domain is an evolutionary conserved protein domain, of about thirty to forty amino-acid residues long, which was found in a large number of mostly animal proteins.Eib and Martens (1996) cloned a novel Xenopus gene, 7365, that is enriched in neuroendocrine tissues. The predicted protein contains a signal peptide, a transmembrane domain, 2 follistatin modules, and an EGF-like domain, a cysteine-rich region first identified in epidermal growth factor. Eib et al. (1998) stated that the unique EGF-like domain and transmembrane and cytoplasmic regions of the human homolog are nearly identical to those found in the mouse and frog homologs.Eib et al. (1998) used somatic cell hybrids and fluorescence in situ hybridization to map the TMEFF1 gene to human chromosome 9q31.
