Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TM6SF2 in samples. An antibody specific for TM6SF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM6SF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM6SF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM6SF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:TM9SF2 contains a putative signal sequence, a hydrophilic N-terminal region, and 9 predicted C-terminal transmembrane domains. TM9SF2 shares 35% amino acid sequence identity with the S. cerevisiae Emp70 protein. By indirect immunofluorescence microscopy, recombinant TM9SF2 appeared to be localized to endosomes by virtue of its apparent colocalization with transferrin receptors and some mannose 6-phosphate receptors; TM9SF2 was not detected in the plasma membrane or in the Golgi apparatus. Northern blot analysis detected an approximately 3.2-kb TM9SF2 transcript in all human tissues tested, with the highest expression in pancreas, high expression in kidney, lower expression in heart, brain, skeletal muscle, and placenta, and the lowest expression in lung and liver.
