Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TM4SF5 in samples. An antibody specific for TM4SF5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM4SF5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM4SF5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM4SF5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Like other TM4SF proteins, TM4SF5 is cysteine-rich. TM4SF5 shares 50% amino acid identity with the tumor-associated antigen L6 (TM4SF1). Northern blot analysis of various normal human tissues, namely intestine, pancreas, stomach, colon, esophagus, bladder, uterus, placenta, and adipose tissue, detected TM4SF5 expression only in intestine and 1 of 14 pancreas samples. TM4SF5 was overexpressed in 13 of 14 pancreatic adenocarcinomas, as compared with both normal pancreatic and chronic pancreatitis tissues. TM4SF5 expression was also found in 2 of 2 gastric cancers, 1 of 2 colon carcinomas, 1 of 1 carcinoma of the papilla vateri, and 1 of 1 soft-tissue sarcoma. TM4SF5 was expressed as an approximately 1-kb transcript in the pancreatic tumors.
