Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TM4SF19 in samples. An antibody specific for TM4SF19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM4SF19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM4SF19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM4SF19 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:TM4SF19 Belongs to the L6 tetraspanin family.Members of the L6 family of membrane proteins, a branch of the tetraspanin superfamily, are overexpressed in tumor cells from many types of cancers. However, direct evidence of their oncogenic activity has not been previously shown.
this gene is expressed at low level, only 16.6% of the average gene in this release.The gene contains 4 different gt-ag introns. Transcription produces one mRNA. There are 3 validated alternative polyadenylation sites. The spliced mRNA putatively encodes a good protein, containing L6 membrane domain, some transmembrane domains, apparently vertebrate specific Proteins are expected to localize in various compartments.
