Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TM in samples. An antibody specific for TM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Thrombomodulin (TM) is the cell surface receptor for thrombin. When occupied, thrombomodulin converts thrombin from a procoagulant protein into the activator of Protein C. Once activated Protein C (APC) has been generated, thrombomodulin acts as a major anticoagulant through its ability to inactivate various blood factors (Va, Va, Xa and Xa). In competing for thrombin binding, thrombomodulin inhibits the proteolytic effect of thrombin in its clotting of fibrinogen, the inactivation of Protein S and the induction of platelet aggregation.
TM is an integral membrane glycoprotein resembling in structure the low-density lipoprotein (LDL) receptor.TM possesses several EGF repeats, of which numbers five and six are responsible for the high affinity binding of thrombin (Kd = 0.5 nM).
