Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TKTL1 in samples. An antibody specific for TKTL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTKTL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TKTL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TKTL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Transketolase (TKT;) is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. As part of a systematic search for differentially expressed genes, Coy et al. (1996) isolated a novel transketolase-related gene, TKTL1, which they called TKR. They isolated transcripts encoding tissue-specific protein isoforms. Comparison with known transketolases demonstrated a TKR-specific deletion mutating 1 thiamine-binding site.
Genomic sequencing of the TKR gene by Coy et al. (1996) revealed the presence of a pseudoexon, as well as the acquisition of a tissue-specific spliced exon, compared to the TKT gene, which maps to 3p14.3.
